OSI is primarily used as an abbreviation and has many meanings:
General
- OSI Pharmaceuticals,an American pharmaceutical company
- OSI Restaurant Partners, the restaurant and entertainment group that includes Outback Steakhouse
- OSIsoft is a software company based out of San Leandro, California
- OSI Systems a company that manufactures security scanners and medical equipment based in California
- Objective Systems Integrators a company providing Operational Support Systems
- Olomouc Summer Institute, an educational program for high-school students
- Ontario Swine Improvement, a pig breeding company based in Ontario, Canada
- Open Space Institute, an organization that seeks to preserve scenic, natural and historic landscapes
- Open Society Institute, a private charitable foundation established by George Soros to promote open societies around the world
- Ordnance Survey of Ireland (Suirbhéireacht Ordanáis Éireann)
- Otto-Suhr-Institut, the political-science institute of the Free University of Berlin
Government
Technology
Entertainment
Fiction
- "OSI", a fictional United States government agency in The Six Million Dollar Man and Bionic Woman television programs
- "Office of Secret Intelligence", a fictional United States government secret agency in the animated series The Venture Bros
People
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