CIO may mean:

• Central Intelligence Organisation, the secret police in Zimbabwe
• Chief information officer, the head of information technology within an organization
• Congress of Industrial Organizations, a United States trade union confederation also known as the Committee of Industrial Organization
• The Creative Industries Observatory, an academic institution based at the University of the Arts London
• The Central Imagery Office, a predecessor organization of the National Geospatial-Intelligence Agency
• Concurrent Input/Output, a file systems feature introduced in JFS in May 2003

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