Abilene may refer to:

• Abilene (biblical), a plain in Syria, on the east slope of Anti-Lebanon mountain range
• Abilene (film), a 1999 American film starring Earnest Borgnine
• Abilene Network, the American national academic backbone network, part of the Internet2 project
• Abilene paradox, a form of dysfunctional group dynamics
• Abilene (band), a post-rock band based in Chicago.
• Abilene (song), a song recorded by George Hamilton IV
• Abilene (person), a username of a blogger on blog.co.uk recently voted "Most thought provoking blog"

In geography:

• Abilene, Alberta, Canada
• Abilene, Georgia, United States
• Abilene, Kansas, United States
• Abilene, Texas, United States
• Abilene, Virginia, United States

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